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Objective To observe the effect of mannatide combined with silver sulfadiazine for burn wound. Methods A total of 128 cases with burn wound in the People's Hospital of Yangquan Suburb were selected in the research and randomly divided into two groups according to the digital table ,with 64 cases in each group.The control group was given mannatide treatment ,the observation group was treated with mannatide combined with silver sulfadia-zine.The burn wound appearance ,burn wound healing and the incidence of adverse reactions were compared between the two groups.Results The improve rate of wound,wound exudate rate,decrustation rate in the observation group were 82.81%,18.75%,62.50%,respectively,which were higher than those in the control group (50%,46.88%, 31.25%,χ2＝14.71,11.48,12.55,all P＜0.01).The immediate pain relief rate ,infection rate,incidence rate of scar formation and wound healing time in the observation group were 90.63%,3.13%,12.50%,(16.85 ±1.63)d, respectively,which were significantly better than those in the control group [62.50%,14.06%,28.13%,(23.64 ± 2.16)d],there were statistically significant differences between the two groups (χ2＝14.11,4.87,4.83,t＝20.07, all P＜0.05).The incidence rate of adverse reactions in the observation group was 3.13%,which was significantly lower than 14.06%in the control group (χ2＝4.87,P＜0.05).Conclusion Mannatide combined with silver sulfa-diazine is safe and effective in the treatment of burn wound.It can improve burn wound appearance and shorten wound healing time,which is suitable for use in clinical practice.
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Objective:To establish the protein content determination method respectively by Folin-Ciocalteu method and Coomass-ie brilliant blue binding method for mannatide oral solution and compare the results. Methods:The limit of detection, linearity, accu-racy, repeatability, recovery and content of Folin-Ciocalteu method and Coomassie brilliant blue binding method were investigated. Re-sults:As for Folin-Ciocalteu method, the limit of detection was 0. 2 μg, the range of linearity was 0-112. 0 μg(r=0. 9990), the av-erage recovery was 105. 2%(RSD=1. 9%), the RSD of accuracy was less than 1%, and the content result of three batches of samples was 47. 45, 58. 34 and 40. 99 μg·ml-1, respectively. As for Coomassie brilliant blue binding method, the limit of detection was 0. 8 μg, the range of linearity was 0-99. 6 μg(r=0. 9980), the average recovery was 102. 0%(RSD=2. 7%), the RSD of accuracy was less than 1%, and the protein content of samples was not detected out by the method. Conclusion:Folin-Ciocalteu method can be used to determine the protein content in mannatide oral solution reliably and efficiently.
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Objective:To establish the protein content determination method respectively by Folin-Ciocalteu method and Coomass-ie brilliant blue binding method for mannatide oral solution and compare the results. Methods:The limit of detection, linearity, accu-racy, repeatability, recovery and content of Folin-Ciocalteu method and Coomassie brilliant blue binding method were investigated. Re-sults:As for Folin-Ciocalteu method, the limit of detection was 0. 2 μg, the range of linearity was 0-112. 0 μg(r=0. 9990), the av-erage recovery was 105. 2%(RSD=1. 9%), the RSD of accuracy was less than 1%, and the content result of three batches of samples was 47. 45, 58. 34 and 40. 99 μg·ml-1, respectively. As for Coomassie brilliant blue binding method, the limit of detection was 0. 8 μg, the range of linearity was 0-99. 6 μg(r=0. 9980), the average recovery was 102. 0%(RSD=2. 7%), the RSD of accuracy was less than 1%, and the protein content of samples was not detected out by the method. Conclusion:Folin-Ciocalteu method can be used to determine the protein content in mannatide oral solution reliably and efficiently.
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Objective To compare two methods in the determination of mannatide for injection .Methods The contents were determined by spectrophotometry and HPLC method .Results In the samples determined by Spectrophotometric Determi‐nation ,soluble in phenol solution and sulfuric acid ,was detected at wavelength 490 nm ,D‐mannose derivatives linear concen‐tration range is 10 .2‐51 μg/ml (r=0 .999 1) ,the average content was 92 .14% ,the average RSD value was 1 .17% .In the HPLC determination , with color spectrum column : Gemini C18 (250 mm × 4 .6 mm , 5 μm ) , mobile phase acetonitrile -0 .02 mol/L ammonium acetate solution (20∶80) ,flow rate:1 ml/min ,the detection wavelength was at 250 nm , column temperature:30 ℃ ,sample size:10 μl detection .The average content ws 83 .47% ,the average RSD value was 0 .65% ,the content of two kinds of methods could effectively determine the D‐mannose .Conclusion The average content for spectrophotometry was higher ,but the phenol was used in detection of special odor ,poisonous ,corrosive ,would cause a cer‐tain risk .HPLC was exclusive ,chromatogram was more intuitive ,on the operator harmfulness and less irritating .A new meth‐od for the determination of D‐mannose was established at the same time .
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OBJECTIVE: To establish a new determination method for free monosaccharides or disaccharides in glycopeptide drugs to eliminate their influence on polysaccharide content assay by colorimetric method adopted by the current national specifications. METHODS: Solid phase extraction column was used for matrix elimination. A high performance anion exchange chromatograghy (HPAEC) in conjunction with pulsed amperometric detector (PAD) was performed on a CarboPac®PA20, using 200 mmol · L-1 sodium hydroxide solution and 1 mol · L-1 sodium acetate solution as mobile phase. RESULTS: Twenty batches of raw materials and preparations of Bozhi glycopeptides, mannatide and polystictus glycopeptide were tested. Free monosaccharides of high content were detected in eight batches of samples, including glucose, sucrose, mannose, and fructose. CONCLUSION: The HPAEC-PAD method is specific, sensitive, rapid and applicable widely, compared with other methods of saccharide detection, thus has good prospect of application and development. The assay methods in the current national specifications of glycopeptides drugs need to be improved.
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OBJECTIVE:To observe the efficacy and safety of spraying mannatide and 5-fluorouracil in anocelia during sur-gery of lung cancer based on the adjuvant chemotherapy. METHODS:Totally 114 patients with lung cancer radical surgery were randomly divided into observation group(59 cases)and control group(55 cases). There were spraying mannatide(40-80 mg)and 5-fluorouracil(500 mg)in the observation group;and nothing drugs were sprayed in control group. The patients higher than phaseⅡ were treated by adjuvant chemotherapy after surgery for continuous 2 cycles,21 d as a cycle. The clinical data was compared, including amount of bleeding and drainage,hospital stay,complications,toxicity,KPS score,body weight changes,immune func-tion,survival rate and recurrence rates between 2 groups. RESULTS:There were no significant differences among the amount of bleeding and drainage,hospital stay and complications in the 2 groups (P>0.05). The nausea,vomiting,leucopenia,the KPS score,improvement of total effective rate,and improvement rate of body mass,immune function,survival rate in 1 and 2 year (s),recurrence rate and time within 2 years in observation group were significantly better than control group,with significant dif-ferences(P<0.01 or P<0.05). CONCLUSIONS:Spraying mannatide and 5-fluorouracil can reduce the toxicity in anocelia during surgery of lung cancer based on the adjuvant chemotherapy,improve the immune function and survival rate,reduce recurrence rate and prolong the recurrence time.
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Objective To observe the clinical effect of interferon -1 b (Yundesu)in the treatment of verru-ca planae.Methods 1 1 0 patients with verruca pianae were randomly divided into observation group and control group,55 cases in each group.The observation group was given Yundesu injection 30 g once every day in first week, and once every other day in another three weeks.The control group was given orally mannatide 1 0ml two times every day.The leision changes of the two groups were observed after 4 weeks therapy.Results The total effective rate of the observation group was 92.7%(51 /55),which of the control group was 72.78%(40 /55),the difference between the two groups was statistically significant (χ2 =7.70,P <0.01 ).No adverse reactions in the two groups were observed.Conclusion The clinical effect of Yundesu is more prominently beneficial to patients with verruca planae.
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Objective To observe the clinical effect of mannatide treatment with burn infection. Methods 100 burn patients admitted to Sichuan Nanchong Central Hospital from May 2009 to July 2012 were randomly divided into control group and treatment group, each group had 50 cases. Both of two groups were given conventional treatment, treatment group were treated with intravenous mannatide,control group were injected recombinant human growth hormone. Results The average length of stay in treatment group was signiifcantly shorter than control group (P=0.042). The clinical total effective rate, serum IgA, IgG, IgM and other indicators of immune function were improved signiifcantly compared with control group (P=0.037). There was no statistical signiifcance in blood protein, transferrin, prealbumin, glucose, insulin, urinary sodium, urinary potassium, chloride and other indicators of patients between two groups. Conclusion Mannatide can effectively enhance immune function, promote wound healing, shorter hospital stay and improve the clinical efifcacy.
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Objective To investigate the effect of Mannatide capsules and Thymic peptide on children with recurrent respiratory tract infections(RRI) and the influnence on their immunity. Methods 160 patients with recurrent respiratory tract infections were randomly divided into the control group and the treatment group,the treatment group were treated with Mannatide capsales and Thymic peptide while the control group with only the routine therapy. Results The effect of the treatment group was over the control one(P＜0.01),two groups of the patients were promoted on Ig level,and the effect of 160 cases in the treatment group was more obvious than that of the control one(P＜0.05). Conclusion Mannatide and Thyrnic peptide applied together are effective in treating children with recurrent respiratory tract infections.